구강암 발생과정에서 Eukaryotic Translation Initiation Factor-5A의 발현
Expression of Eukaryotic Translation Initiation Factor-5A in Oral Carcinogenesis
이상임, 이준, 이선경, 배원정, 김선주, 김영일, 최인석, 이승훈, 이상광, 이화준, 박제상, 김은철,
소속 상세정보
이상임 ( Lee Sang-Im ) - 원광대학교 치과대학 구강악안면병리학교실
이준 ( Lee Jun ) - 원광대학교 치과대학 구강악안면외과학교실
이선경 ( Lee Sun-Kyung ) - 원광대학교 치과대학 구강악안면병리학교실
배원정 ( Bae Won-Jung ) - 원광대학교 치과대학 구강악안면병리학교실
김선주 ( Kim Sun-Ju ) - 원광대학교 치과대학 구강악안면외과학교실
김영일 ( Kim Young-Il ) - 원광대학교 치과대학 구강악안면병리학교실
최인석 ( Chio In-Suk ) - 원광대학교 치과대학 구강악안면병리학교실
이승훈 ( lee Seung-Hoon ) - 원광대학교 치과대학 구강악안면병리학교실
이상광 ( Lee Sang-Kwang ) - 원광대학교 치과대학 구강악안면병리학교실
이화준 ( Lee Hwa-Jun ) - 원광대학교 치과대학 구강악안면병리학교실
박제상 ( Park Jae-Sang ) - 원광대학교 치과대학 구강악안면병리학교실
김은철 ( Kim Eun-Cheol ) - 원광대학교 치과대학 구강악안면병리학교실
KMID : 0829220090330030129
Abstract
Eukaryotic translation initiation factor 5A (eIF-5A) is essential for proliferation of eukaryotic cells, andwas identified as diagnotic marker in cervical intraepithelial neoplasia, cervical and endometrial cancer, but relatively little is known about thein vivo and in vitro expression patterns of eIF-5A in oral premalignant and malignant lesions mirror the expression levels observed in vitro with cells derived from normal oral mucosa, immortalized oral keratinocytes (IHOK) and primary and metastatic oral squamous cell carcinoma (OSCC). We used an oral squamous cell carcinoma (OSCC) progression model composed of cell lines and tissue specimens to characterize expression patterns by Western blotting and immunohistochemistry. eIF-5A and PCNA levels are elevated in IHOKand primary and metastatic OSCC cella as compatred to normal human oral keraitinocytes. eIF5A and PCNA expression was limited to basal cells of normal oral mucosa. eIF-5A and PCNA expression is increased in dysplastic epithelium spreading to more superficial layers, and its expression levels correlated significantly with the degree of dysplasia. Well and moderately differentiated OSCC showed strong expression of eIF-5A and PCNA. These results suggest that upregulated expression of eIF-5A seems to be an important epigenetic alteration that accompanies oral carcinogenic progression, and eIF-5A could be used as an biomarker for oral premalignat lesion or squamous cell carcinoma.
키워드
Oral cancer;Immortalized keratinocytes;Precancerous lesion;EIF-5A; Cancer marker
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